| 質粒名稱: | miR155 in pcDNA3.1 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 78126 |
| 存儲實驗室: | Heidi Schwarzenbach |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3.1 |
| 載體出品公司: | Invitrogen |
| 完整質粒大小: | 4989 bp |
| 載體類型: | 哺乳細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | miR155 |
| 物種來源: | H. sapiens (human) |
| 啟動子: | CMV |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | XhoI (not destroyed) |
| 3’克隆位點: | XhoI (not destroyed) |
| 5’測序引物 | CMV-F |
| 3’測序引物 | BGH-Rev |
|
文獻引用方法 |
|
| 材料和方法部分: | miR155 in pcDNA3.1 was a gift from Heidi Schwarzenbach (Addgene plasmid # 78126 ; //n2t.net/addgene:78126 ; RRID:Addgene_78126) |
| 參考文獻部分: | Increased serum levels of circulating exosomal microRNA-373 in receptor-negative breast cancer patients. Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, Pantel K, Schwarzenbach H. Oncotarget. 2014 Oct 30;5(20):9650-63. 10.18632/oncotarget.2520 PubMed 25333260 |
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miR155 in pcDNA3.1載體質粒基本信息
miR155 in pcDNA3.1質粒圖譜載體圖譜和miR155 in pcDNA3.1載體序列質粒序列多克隆位點信息
miR155 in pcDNA3.1質粒載體簡介
miR155 in pcDNA3.1質粒序列載體序列
LOCUS miR155 in pcDNA3.1 5504 bp DNA circular SYN 14-APR-2025
DEFINITION Mammalian expression of miR155.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5504)
AUTHORS Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H,
Pantel K, Schwarzenbach H
TITLE Increased serum levels of circulating exosomal microRNA-373 in
receptor-negative breast cancer patients.
JOURNAL Oncotarget. 2014 Oct 30;5(20):9650-63.
PUBMED 25333260
REFERENCE 2 (bases 1 to 5504)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Oncotarget."; date: "2014-10-30"; volume: "5(20)"; pages: "9650-63"
COMMENT Sequence Label: miR155 in pcDNA3.1
FEATURES Location/Qualifiers
source 1..5504
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1098..1115)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1104..1328
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1374..1802
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1461..1480)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1671..1692
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(1811..1831)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 1816..2145
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1996..2131
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2058..2077
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2212..3006
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2266..2285)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 2876..2895
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3180..3301
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3217..3236)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3271..3290
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3350..3366)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3350..3366)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3363..3385)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3374..3390
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(3398..3404,3405..3422,3423..3428))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3443..3464
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3581..3598)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3752..4337)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3832..3851)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4508..5299,5300..5368))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5131..5150
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5369..5473)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
961 agatatccag cacagtggcg gccgctcgag atctctttaa tgctaatcgt gataggggtt
1021 tttgcctcca actgactcct acatattagc attaacagtc ctcgagtcta gagggcccgt
1081 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
1141 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
1201 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
1261 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
1321 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
1381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
1441 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
1501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
1561 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
1621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
1681 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
1741 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
1801 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
1861 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
1921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
1981 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
2041 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
2101 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
2161 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
2221 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
2281 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
2341 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
2401 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
2461 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
2521 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
2581 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
2641 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
2701 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
2761 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
2821 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
2881 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
2941 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
3001 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
3061 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
3121 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
3181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
3241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
3301 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
3361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
3481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
3541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
3601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
3661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
3721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
3781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
3841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
3901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
3961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
4081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
4141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
4201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
4261 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
4321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
4381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
4441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
4501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
4681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
5041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
5101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
5161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
5401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
5461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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